Fluorescent In Situ Hybridization Principle

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Fluorescent in situ hybridization principle. A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of or in addition to scattering reflection and attenuation or absorption to study the properties of organic or inorganic substances. To build comprehensive models of cellular states and interactions in normal and diseased tissue genetic and proteomic information must be extracted with single cell and spatial resolution. This testing method is a type of immunoassay.

It is based on the principle that antibodies will bind to very specific antigens to form antigen antibody complexes and enzyme linked antigens or antibodies can be used to detect and measure these complexes. After a diagnosis of metastatic breast cancer has been made her 2neu testing may be used as a prognostic marker to help determine how aggressive the breast cancer tumor is likely to be. Fluorescence in situ hybridization fish is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence complementarity.

There are two general approaches to diagnosing condition diseases or conditions by immunoassay testing for specific antigens or for antigen specific antibodies. Access to tables of contents and abstracts is complimentary while access to articles is limited to print subscribers. In this section some commonly used virological methods will be discussed in further detail.

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